Isolation of the Principal Neurotoxins of Two Naja naja Subspecies

Identification and isolation of immunodominant proteins of Naja naja (Oxiana) snake venom

Snake venom is a complex mixture of proteins, peptides, enzymes, carbohydrates, and minerals. They contain a variety of chemicals with pharmacological and toxicological properties. The innate immune system is the first line of defense against toxins and microbes. Antibacterial and anticancer proteins produced by snake venom have recently attracted significant attention due to their relevance to...

Cardiotoxin III from the Taiwan cobra (Naja naja atra). Determination of structure in solution and comparison with short neurotoxins.

The structure in solution of cardiotoxin III, a membrane toxin purified from the venom of the Taiwan cobra, Naja naja atra, is reported. Sequence-specific assignment of 1H-NMR lines was completed and the NMR data show the presence of a triple and a double-stranded antiparallel beta-sheet. Many NOE cross peaks identified in NOESY spectra were applied as distance constraints based on a hybrid dis...

Chemical Modification of the Histidine Residue in Phospholipase A, (Naja naja naja)

Reaction of phospholipase A, (Naja naja naja) with pbromophenacyl bromide leads to almost complete loss of enzymatic activity. The rate of inactivation is pa-dependent with ,pK, = 6.9 for the ionizing residue. p-Bromophenacyl bromide modifies 0.5 mol of histidinelmol of enzyme as judged by amino acid analysis and incorporation studies with W-labeled reagent. The rate of inactivation is affected...

Isolation of phospholipase A isoenzymes from Naja naja venom and their action on membrane-bound enzymes.

CHARACTERIZATION OF AN ANTICOAGULANT AGENT IN THE VENOM OF IRANIAN COBRA (NAJA NAJA OXIANA).

Snake venoms contain a rich variety of factors which affect blood coagulation. There have been few reports on the anticoagulant activity of the venom of different cobras, but no such observations have been made on the Iranian cobra, although a procoagulant factor has been described. In this study an anticoagulant factor has been purified from the venom of Naja naja oxiana using gel filtrat...